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UID:20260626T020959EDT-10391l17S4@132.216.98.100
DTSTAMP:20260626T060959Z
DESCRIPTION:PhD Oral Defence of Ejimedo Madogwe\, Animal Science\n\nInferti
 lity affects 1 in 6 couples in Canada. Infertility also affects animal pro
 duction systems resulting in reduced efficiency and profitability. Fertili
 ty is a result of efficient ovarian follicular growth and successful ovula
 tion\, which are regulated by the pituitary gonadotropins\, follicle stimu
 lating hormone (FSH) and luteinizing hormone (LH). Gonadotropins regulate 
 ovarian functions through signaling pathways such as extracellular signal 
 regulated kinases (ERK1/2) pathway and unique gene expression programs. Hi
 stone modifications such as trimethylation of lysine 4 of histone H3 (H3K4
 me3)\, a histone mark associated with active transcription\, are involved 
 in gene expression. We hypothesized that FSH and LH regulate follicular de
 velopment and ovulation by instigating specific gene expression programs t
 hrough the ERK1/2 pathway and H3K4me3. We used mouse models to test this h
 ypothesis. Using RNASequencing\, we explored the FSH and LH regulated tran
 scriptome of granulosa cells. We used chromatin immunoprecipitation and se
 quencing (ChIP-Seq) to identify H3K4me3 enrichment in the promoter of FSH 
 and LH regulated genes. Combination of these techniques with pharmacologic
 al inhibition of ERK1/2 signaling revealed ERK1/2 dependent genes in granu
 losa cells and that ERK1/2 signaling plays a role in H3K4me3 deposition in
  the promoters of LH-regulated ovulatory genes. Finally\, we discovered th
 at sustained ERK1/2 signaling uniquely regulates follicular rupture\, with
 out affecting other processes of ovulation such as oocyte meiotic resumpti
 on and luteinization. Overall\, these studies provide molecular evidence t
 hat FSH and LH regulate ovarian follicular development and ovulation throu
 gh histone modification in ovarian granulosa cells.\n\nEveryone in the McG
 ill community is welcome to attend a PhD Oral Defence. Please join us in c
 elebrating the accomplishments of our PhD candidates.\n\n \n\n \n\n \n
DTSTART:20200323T131500Z
DTEND:20200323T131500Z
LOCATION:MS2-022\, Macdonald-Stewart Building\, CA\, QC\, St Anne de Bellev
 ue\, H9X 3V9\, 21111 Lakeshore Road
SUMMARY:PhD Oral Defence: Gonadotropin regulation of granulosa cells throug
 h follicular development and ovulation
URL:/macdonald/channels/event/phd-oral-defence-gonadot
 ropin-regulation-granulosa-cells-through-follicular-development-and-321102
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