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UID:20260624T053712EDT-4603B2xidW@132.216.98.100
DTSTAMP:20260624T093712Z
DESCRIPTION:Bioinformatics Seminar Series\nDr. John Bergeron\nAbstract: The
  objective of a human proteome\nproject is to characterize quantitatively 
 a representative protein\nfor each protein coding gene in its major organ 
 and intracellular\nsite of expression in the context of its major protein 
 partners.\nThe concept of a single representative protein for each protein
 \ncoding gene enables a gene centric approach to be implemented for\nthe c
 ompletion of the human proteome. A recently annotated human\ngenome sugges
 ts ca 20\,500 protein coding genes of which ca 8\,000\nhave no direct evid
 ence for a protein expressed and a further 5\,000\nwhere the evidence may 
 be weak. Hence a human proteome project will\nprovide the missing evidence
  for protein expression by predicted\nprotein coding genes. Current effort
 s to define the proteome using\nantibodies monospecific to a representativ
 e protein for each\nprotein coding gene are ongoing with almost a third of
  the human\ngenome mapped in this way via the Human Protein Atlas. Current
  mass\nspectrometry efforts have enabled community standards to be\nimplem
 ented via test samples. The extension to label free methods\nto quantify p
 roteins by tandem mass spectrometry as well as their\nquantitation using a
  serial dilution of heavy isotope labelled\npeptides diagnostic of each re
 presentative protein provides the\nframework to define protein abundance f
 or each representative\nprotein in its major site of expression. Using bot
 h tagged genes\nfor expression in mice and human cell lines\, a protein in
 teraction\nmap may be defined for each representative protein and confirme
 d by\ncoimmunoprecipitations with a polyclonal antibody resource followed
 \nby tandem mass spectrometry to characterize protein partners.\nComputati
 onal biology to merge the data from the antibody directed\nmapping of the 
 proteome along with the quantitative estimates of\nprotein abundance via t
 andem mass spectrometry and protein partners\nalso deduced by tandem mass 
 spectrometry defines the resource of a\nhuman proteome.\n
DTSTART:20080924T183000Z
DTEND:20080924T193000Z
LOCATION:McIntyre Medical Building\, CA\, QC\, Montreal\, H3G 1Y6\, 3655 pr
 omenade Sir William Osler
SUMMARY:Human Proteome Project
URL:/channels/event/human-proteome-project-101851
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